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As described previously ( 2.12 Erythrocyte membrane preparation Erythrocyte membranes were prepared as previously described ( 2 O 2 in the presence or absence of ME, erythrocyte membrane proteins were isolated using cold hemolysis buffer (2.5 mM NaH 2 PO 4 ) supplemented with protease and phosphatase inhibitors (1 mM PMSF, 1 mM NaF, and 1 mM sodium orthovanadate)
This is also the case for those with exocrine pancreatic insufficiency (EPI)
The peptide doesnt colour the skin directly
The FPA value was determined by measuring the absorbance at an excitation wavelength of 485 nm and emission wavelength of 528 nm and calculated according to the following formula: In competitive inhibition experiments, increasing concentrations of inhibitor peptides were titrated with a mixture of 120 nM FHD and 10 nM FITC-p53 TAD PEP
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