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We tested whether COP1 or DDD-COP1 directly interacts with p53 using co-expression and pull-down assays in human cells, and found no detectable interaction under the conditions tested (Supplementary Fig

In additional studies, Sekhars group found that deficient mitochondrial fat metabolism was linked to glutathione deficiency, and that correcting glutathione deficiency also improved mitochondrial fuel burning function in aging, as published in the Aging Cell

This lack of specificity complicates precise evaluation of the pharmacological and toxicological effects attributable to particular preparations

Here is how to handle the most common problems researchers encounter