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Trx2 also induces apoptosis via ASK1 when being oxidized, as well as giving rise to increased ROS levels in the mitochondria, leading to release of cytochrome c and activation of caspase 3 and 9 46

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It can not only simulate the internal environment of the human body but also provide a platform for accurate evaluation of the toxicity of TCMs, reveal the mechanism of action of the toxic components of TCMs, and provide a scientific basis for the safe use of TCMs

NADPH dependence of inhibition TGR was incubated at room temperature with inhibitor in the presence or absence of 100 M NADPH for 15 min