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On day 21, tumor progression was evaluated using bioluminescence imaging with the IVIS system (Caliper Life Sciences) following intraperitoneal injection of D-Luciferin potassium salt (3 mg/kg per mouse, administered 5 minutes prior to imaging, Beyotime, ST198) and anesthesia with 1% pentobarbital sodium (80 mg/kg per mouse)

For SC injections, the medication is placed in the soft, fatty tissue directly beneath the skin, with the needle angled at roughly 45 degrees

One is that research has yet to prove Lipo-C injections safe or effective

By upregulating key genes such as interleukin-17 (IL-17) and retinoic acid receptor-related orphan receptor gamma t (RORt), which are essential for Th17 cell differentiation and inflammation regulation, STAT3 effectively amplifies the inflammatory cascade reaction (Atreya et al., 2000