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Histological analysis All tissues/organs were examined for histopathological changes, which were diagnosed following the International Harmonization of Nomenclature and Diagnostic Criteria (INHAND), Society of Toxicological Pathology (www.toxpath.org/inhand.asp)
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Plasmids were amplified by incubation of lysogeny broth (LB) cultures containing the appropriate antibiotics overnight at 37 C
Liver-derived acylcarnitine can be used as a signal molecule for inter-tissue communication to coordinate energy metabolism, highlighting the role of the liver as a metabolic center, and unveiling the new insight of the liver-organ axis
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