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Suggested benefits for mood, cognition, and possibly conditions like traumatic brain injury, Parkinsons disease, and depression, but these claims are not substantiated in humans358
The signal is important, but it is not proof yet
Novo Nordisk funded the study
Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells
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