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McFarlan SC, Terrell CA, Hogenkamp HP

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SKOV3 cells were cultured in Gibco RPMI media 1640 (ThermoFisher, Paisley, UK) supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin (ThermoFisher, Paisley, UK) at 37 C with 5% CO 2 and balanced with air

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Recent evidence suggests that progression to type 2 diabetes results not only from insulin decreased insulin sensitivity (insulin resistance) but also from loss of insulin secretion

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