The pancreas was then dissected, incubated at 37C for 10 minutes, and digestion halted with cold RPMI-1640 supplemented with 10% FBS (F7524, Sigma-Aldrich) and 1% (v/v) penicillin/streptomycin solution (15070-063, Invitrogen)
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This will ensure a smooth recovery and optimise your results
Appl Microbiol