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The priming phase is initiated when DAMPs or PAMPs engage receptors such as toll-like receptors (TLRs) or cytokine receptors, triggering the NF-B pathway to increase mRNA levels of NLRP3, pro-IL-1, and pro-IL-18 (Zahid et al., 2019)
Samples were then washed with PBS as described previously and incubated with goat anti-rabbit secondary antibody (1:500 dilution, Beyotime) at room temperature for 1 h in darkness
Research suggests GHK-Cu can influence the expression of genes involved in tissue repair, inflammation regulation, antioxidant defense, and extracellular matrix remodelingthe network of proteins and structural components that helps skin remain strong, resilient, and youthful
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