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Every time you pierce the rubber stopper with a needle to draw a dose, you introduce a potential contamination pathway
Homogenization and biochemical analyses Kidney homogenates (10% w/v) were prepared in ice-cold (4 o C) TrisHCl, pH 7.4 buffer in a homogenizer fitted with a Teflon pestle (Glas-Col, Vernon Hills, IL, USA) and homogenized at 8001000 r.p.m
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PMID: 93 HirkoKARocqueGReasorETayeADalyACutressRIet al
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