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Research utilizing the Broad Institute's Connectivity Map indicates it influences the expression of over 4,000 genes (~31% of the human genome), shifting gene signatures from a pathological state back to a healthier, regenerative baseline
For overexpression studies, SGBS cells were transduced with doxycycline-inducible lentiviral vectors expressing GFP or GPR146 and treated with 1 g/mL doxycycline during the experimental period as indicated
On a cellular level, conjugates have been shown to be subjected to deconjugation by enzymes such as glucuronidases bound to the cell surface
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