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Graphical Abstract Highlights Oocystis borgei can effectively remove copper ions from seawater, with an average removal rate of 82% RNA-seq revealed 1512 DEGs under Copper stress indicating profound transcriptomic remodeling Copper exposure upregulated genes linked to transport, redox balance, photosynthesis and GSH Fingdings uncover molecular basis of algal copper tolerance and support bioremediation Access this article Similar content being viewed by others Data Availability The raw sequence data can be accessed at NCBI-SRA using the accession number PRJNA1116067
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