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The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min
Available at: (Accessed August 10, 2022)
This schedule also reduces the chance that magnesium supplementation will interfere with appetite suppression benefits during the day
This hormone and the medication that mimics it helps regulate blood sugar levels
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