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A 2014 study by Nakajima, Nagata, and colleagues published in Archives of Dermatological Research showed that reduced glutathione disrupts intracellular trafficking of tyrosinase to melanosomes, the rate-limiting enzyme in melanin synthesis, attenuating melanization at the cellular level
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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min
Critical role of GSH in silica-induced oxidative stress, cytotoxicity, and genotoxicity in alveolar macrophages
[2] [3] This mechanism has several physiological effects that contribute to glycaemic control, with appetite reduction and weight loss occurring as secondary effects
