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2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes
When zinc is low, the activity of two key enzymes, glutamate cysteine ligase (GCL) and glutathione reductase, goes down
This is a significant finding as it suggests that the normal cells treated with epitalon will not gain full immortality and an extended lifespan as seen in cancer cells
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