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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

This stability is attributed in part to the presence of proline residues, which help protect the peptide from enzymatic breakdown and support its potential as a research chemical in studies involving the gastrointestinal tract and other tissues

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