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Then, the cell viability was determined, and 100 L supernatant from cell culture medium was collected to measure LDH content using the specific kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturers protocol [28]
Oral administration of tadalafil (20 mg) or sildenafil (100 mg) was given
At the same time, it reduces the release of glucagon, another hormone involved in raising blood sugar
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