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Currently, there are multiple methods for obtaining ESCs, such as activating unfertilized oocytes to develop into parthenogenetic embryos, injecting sperm into enucleated oocytes to generate androgenetic embryos, and transferring somatic cell nuclei into enucleated oocytes to induce reprogramming and establish ESC lines (Leeb and Wutz, 2011
Mohamad N-V, Razali N-SC, Shamsuddin N-AM
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