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A cross-linking step [2 mM 3,3-Dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP, Sigma-Aldrich) for 30 minutes at room temperature followed by quenching with 20 mM Tris HCl for 15 minutes and 3 PBS washes] was performed prior to cell lysis for the SPHKAP-EGFP co-immunoprecipitation experiments to preserve protein-protein interactions despite proteolytic cleavage of the full-length fusion protein during the IP process
Specifically, treated granulosa cells exhibit reduced reactive oxygen species and enhanced mitochondrial membrane potential, both of which are associated with oocyte health
Compounds and treatment groups Grouping and dosing for the study are listed in Supplementary Table S2, and a schema of the design is shown in Supplementary Fig
Mohammad Aqueel Ahmed Dr
