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Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3-diaminobenzidine substrate (ab64238, Abcam) for 10 min
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It also plays a key role in converting protein and fat into energy
Taking advantage of these resources before considering peptide use establishes baseline health status and increases confidence that no occult malignancy exists
