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Other common mistakes include shaking instead of gently swirling, spraying solvent directly onto fragile powder, using an unlabeled vial, exposing the solution to light, storing it in the refrigerator door, and confusing sterile water with bacteriostatic water

After incubation, cells were stained with propidium iodide (PI, # 40710ES03, Yeasen) solution (40 g/mL in PBS) for 15 min at room temperature and the cell cycle was analyzed using Flowsight flow cytometer (Merck Millipore, Darmstadt, Germany)

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