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Co-immunoprecipitation (Co-IP) After treatment with the indicated compounds, cells were harvested with 50 l of IP lysis buffer (50 mM Tris HCl, pH 7.5, 100 mM NaCl, 2 mM EDTA, 5% glycerol, 0.5% Triton X-100) supplemented with protease/phosphorylase inhibitors for each well of the 6-well plate and lysis was performed by incubation for 30 min on ice
By 12 weeks on the medication, that initial fatigue is generally resolved, according to the International Association for Physicians in Aesthetic Medicine
Arul, V., et al
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