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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker
Presented for research literature review only Murata T et al
Because most evidence is still preclinical or anecdotal, symptom tracking is more useful than expecting a fixed timeline
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