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Around 4 l of the mixture of 0.02 mg/ml TGR in 0.15% DMSO and 5 mM inhibitor was applied to home-made carbon film evaporated a mica film, which was floated off in about 200 l of 2% sodium silicotungstate (SST) and recovered by a Cu grid
Sphincter recovery often occurred alongside improvement in brain lesions and normalization of intracranial pressures in multi-system injury models
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