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doi: 10.1186/s13075-018-1611-2 102 IsozakiTArbabASHaasCSAminMAArendtMDKochAEet al

Samples were first incubated with a primary antibody at 4 C overnight followed by repeated washing and then incubating with a secondary antibody and 100 ng/mL of 4,6-diamidino-2-pheny-lindole (DAPI) for 1 h

Serum phosphorus levels should be monitored monthly after starting MD, and dietary phosphorus restriction should be employed with use of phosphate binders as necessary to ensure that serum phosphorus remains within the normal range

Transfecting TREM2 into the cells was found to reverse this phenomenon, while knocking out TREM2 in mice increased the levels of TNF-, IL-1 and IL-6 and decreased the levels of IL-10