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For western blot analyses, the protein content of the lysosomal-enriched gradient supernatant was quantified using a Qbit 1 fluorometer (Thermo Fisher Scientific) and a protein quantification kit (Thermo Fisher Scientific, Q33212)
TB-500's parent Thymosin -4 has been characterised across actin-cytoskeleton dynamics, cell migration, cardiac progenitor mobilisation, and corneal wound-healing in foundational and contemporary research (PMID 11579089, PMID 15565145, PMID 11950239)
Free Radic Biol Med 73:366382
Glycol solvents (propylene glycol, propanediol, pentylene glycol) are generally well tolerated on intact skin but are a more common sensitivity trigger than a simpler carrier, and are worth avoiding for anyone already managing a reactive skin condition
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