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According to one comprehensive analysis, knee OA has been sharply on the rise over the past three decades, with over 3 million cases reported world-wide in 2021, compared to only 1.4 million in 1990
For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35
Technique: Roll the device across thinning areas in multiple directions, longitudinal, vertical, and diagonal
The sealed vial is protected against moisture do not open until ready to reconstitute
