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Reduction of roGFP2-SG by GSH is the rate limiting step which is accelerated by GRXs, with its reactive cysteine attacking the disulfide of roGFP2-SG, resulting in glutathionylated GRX (GRX-SG) and roGFP2
Detection of intracellular glutathione The content of total glutathione in cell lysates were determined using an enzymatic recycling method
Tirzepatide dosing follows its own escalation schedule: 2.5 mg for weeks 1 through 4, then 5 mg, 7.5 mg, 10 mg, 12.5 mg, and up to 15 mg at maintenance
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