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Comprehensive laboratory testing is performed before treatment initiation and at regular intervals
Injecting air before drawing solution introduces atmospheric contaminants and increases oxidation exposure
WT-boxes occurred much less frequently in GST promoters (12 copies) than W-boxes (18 copies)
The 50:10:10:10 ratio ensures effective metal-ligand complexation across the reconstituted volume and reflects the compositional standard documented in published KLOW formulation literature
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