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Utilizing 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, equivalent quantities of protein, 30 g of total protein for each lane, underwent electrophoresis and then transferred to a polyvinyl difluoride membrane (Millipore, USA) applying semi-dry transfer methods (Towbin et al., 1979)
Supports integrated longevity pathway investigation: Aging is influenced by interconnected processes including telomere shortening, oxidative stress, and circadian disruption
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