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10.1016/j.pharmthera.2006.11.007 39 DuarteA

This is the process where the liver takes fat-soluble toxins and turns them into water-soluble compounds that the body can easily flush out

Then, the transwell membrane was fixed by adding 4% paraformaldehyde PFA to both chambers for 30 min at 25 C, followed by washing and cryopreservation the membrane with a series of sucrose solution (10% to 30%)