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Recent studies have shown that ghrelin receptor antagonism inhibits GLP-1 production induced by oral glucose challenge (47, 48)
Q-PCR reaction mixtures contained 1 l cDNA, 1x SYBR Green Supermix (BioRad, Hercules, CA), and 0.15 M gene-specific primer sets in a total volume of 25 l
This approach more closely mimics the body's natural, rhythmic release, creating distinct peaks and troughs in hormone levels
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