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The resin was packed into a glass column and washed with 20 column volumes of 20 mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 5mM CaCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS followed by elution with buffer containing 5mM EGTA and 0.1mg/ml FLAG peptide