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Sections were deparaffinized in a xylene ethanol series and placed in Tris-EDTA (TE) buffer for antigen retrieval (10 mM Tris, 1 mM EDTA, 0.05% Tween, pH = 9.0) and then blocked in 5% BSA
NTS (240) were transferred to U-bottom 96 well assay plates containing 200 l of M199 medium supplemented with 5.5 mM D-glucose, penicillin-streptomycin and 5% heat inactivated fetal bovine serum and incubated at 37 C in a 5% CO 2 incubator overnight
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