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The primers used for cloning are shown in Supplementary Data 5

10 mg vial reconstitution math BAC water added 1.0 mL Final concentration 10 mg/mL Research amount 1 mg Draw volume 0.10 mL U-100 syringe units 10 units BAC water added 1.0 mL Final concentration 10 mg/mL Research amount 2.5 mg Draw volume 0.25 mL U-100 syringe units 25 units BAC water added 1.0 mL Final concentration 10 mg/mL Research amount 5 mg Draw volume 0.50 mL U-100 syringe units 50 units A 5 mg amount uses half of this vial and requires a 0.50 mL draw at this concentration

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doi: 10.1038/s12276-024-01214-1 70 WangHZhengXLiuBXiaYXinZDengBet al