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FLAG-TRIP12 and FLAG-UBR5 were purified from 293 T cells using anti-FLAG antibody (Sigma-Aldrich, M2) and eluted using 200 ng/L of FLAG peptide
glimepiride, where the improvement in histological features of NAFLD in diabetic patients will be measured (NCT02649465)
Conventional Treatment Adequate fluid and fiber are first-line treatments, and regular exercise is also an important intervention Laxatives (osmotic and stimulant), suppositories, and enemas Note: Chronic use of stimulant laxatives may damage the neuromuscular system of the colon, worsening constipation and leading to dependence
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