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Cells were washed with DPBS, harvested (on ice), centrifuged (200 g, 5 min, 4 C), and the resulting cell pellets were immediately frozen in a dry ice/ethanol bath

Conserved proteins as immunogens: glutathione S-transferase of Schistosoma [PMID: 15275483] Laboratory literature summary: Conserved proteins as immunogens: glutathione S-transferase of Schistosoma

Subgroup analyses showed no difference in adverse event rates or weight loss efficacy between supplement users and non-users