As a central transcription factor, NRF2 influences this cell death mode by inducing a variety of genes including SLC7A11, GPX4, FSP1, NQO1, HMOX1, ferritin heavy chain 1 (FTH1), HECT and RLD domain-containing E3 ubiquitin protein ligase 2, and vesicle-associated membrane protein 8 in hepatocellular carcinoma and ovarian cancer cells [85, 86]
After 24, 48 or 72 h exposure to experimental conditions, the conditioned culture medium (supernatant) was collected, and adherent cells were detached with 0.05% trypsin-EDTA
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