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Eluate incubated with TEV protease was dialysed against 25 mM Tris (pH 7.5), 150 mM NaCl and 1 mM DTT overnight at 4 C, then incubated with Ni-NTA resin for 1 h to remove the His-tagged protease
The use of eryptosis in the assessment of toxic parent compounds and their metabolites Eryptosis studies can also be used to compare parent compounds with their metabolites
Several case studies have shown a relationship between KD and PP (Table 3)
The disorder results from pathogenic variants in either of two members of the WNK [ W ith N o ( K )lysine] kinases, WNK1 and WNK4
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