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Materials and Methods Animals Pathogen-free female C57BL/6 mice (1012 weeks old) were obtained from Charles River and housed in rooms maintained at constant temperature and humidity with a 12 hours light cycle

These factors determine how well the vitamin is absorbed, stored, and utilized

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Overview of amino acid metabolism Amino acids are organic compounds containing amino and carboxyl groups, which can be divided into -, -, -, - amino acids according to the position of the functional groups of the core structure, the most important of which are the 22 alpha-amino acids that make up proteins and 20 of these amino acids are involved in protein synthesis