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Oxidation of roGFP2 WT resulted in an increased fluorescence at an excitation of 400 nm and a decreased fluorescence at an excitation of 484 nm as reported previously 66
Patience is a virtue in the lab
Inhibition of NGLY1 inactivates the transcription factor Nrf1 and potentiates proteasome inhibitor cytotoxicity
After air-drying, the incorporation of [- 32 P] was quantified using a LS6500 multi-purpose liquid scintillation counter (Beckman Coulter, Brea, CA) via Cerenkov counting
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