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HILIC provides complementary information to RPLC: usually offering higher retention for polar analytes that typically have poor retention and often elute near the void volume in RPLC mode

observed no differences in visual field improvement over 12 months between the GLP-1 group and controls both groups improved comparably under acetazolamide, reflecting that adding a GLP-1 RA did not yield an extra short-term visual field gain beyond standard care [38]

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