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Insert it into the MT2 vial through the centre of its grey cap which was below the yellow cap (there is a circle raised top of the grey cap use this as a guide for the centre, if you feel resistance you may need to place the needle slightly off centre), Aim the needle part of the syringe at the side of the vial and depress the plunger slowly to fill the vial with 1ml of water
Supplements provide the critical biochemical tools your cells need to repair themselves, but you must also provide the environment of radical rest required for that repair to take place
A Seahorse XFe96 Analyser (Agilent Technologies) was used to assess mitochondrial complex-specific OCR of HCT116 CRC cells
I did this purposefully because I would inject at midday, and then I would go outside and lay out for 30-60 minutes
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