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The mixture was incubated at room temperature for 15 min, followed by adding 1 mL of diluted PEG (0.8 mL of PEG + 0.2 mL of TE/Cation Kit components) and incubated at room temperature for another 3 h
Previous Gastric Surgery: Procedures like gastric bypass can reduce the stomach's ability to produce intrinsic factor and absorb B12
Lyophilization puts the peptide into a state of suspended animation
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