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Assessment of H188 subcellular localization in HEK293-H188-C24 cells Live-cell imaging to detect H188 subcellular localization was performed at 23 C using a Zeiss Axio Observer.Z1 microscope equipped with 100x or 63x Plan-Apochromat Oil DIC objectives (Carl Zeiss, Inc., Thornwood, NY) and interfaced with a Hamamatsu Orca-Flash4.0 LT camera (Hamamatsu Photonics, Bridgewater, NJ), a PhotoFluor LM-75 light source (89 NORTH, Burlington, VT), and an FITC filter set (Chroma Technology Corp.)
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Inhibition of selenoprotein synthesis by selenocysteine tRNA[Ser]Sec lacking isopentenyladenosine
