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The plates were sealed with AeraSeal films (Merck, A9224) to allow gas exchange in cultures and incubated at 30 C, 225 rpm and 80% relative humidity in a humidity shaker (Minitron, INFORS)

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Individuals who may warrant closer monitoring include: Those with a personal or family history of depression , anxiety, or other mood disorders People who have previously experienced mood changes on other weight-loss medications or GLP-1-based therapies Individuals with eating disorders or a complex relationship with food , for whom appetite suppression may have psychological implications (see NICE guideline NG69: Eating disorders: recognition and treatment) [10] Those experiencing significant life stressors alongside starting a new treatment People with poorly controlled type 2 diabetes , where fluctuating blood glucose levels both hypoglycaemia and hyperglycaemia can independently affect mood, concentration, and emotional wellbeing It is also worth acknowledging that obesity itself is associated with higher rates of depression and anxiety, and that the psychological experience of rapid body weight change whether positive or negative can be emotionally complex

Compounding pharmacies operate under state pharmacy board oversight, federal compounding law (DQSA), and for those that pursue it voluntary PCAB accreditation that sets standards beyond what state licensure requires on its own