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At the end point, cells were rapidly washed with ice-cold PBS and incubated at 80 C for 30 min with extraction solvent at a ratio of 1 ml per 1 10 6 cells
J Biotechnol 304:2830
Another control-HGF group ( light-blue bars 5 , receiving a solvent followed by the wash-out step) also displayed a low activation index comparable to the level of the control HGF ( gray bars 3 ), indicating again that the solvent of LASSS is not involved in the mechanism that elevates c-met binding, as shown in Figure 1e, right column
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