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Imai H, Matsuoka M, Kumagai T, Sakamoto T, Koumura T
Why this matters: Higher nerve density - The inner thigh has more nerves, making injections more painful Blood vessel proximity - The femoral artery and vein run through this area, increasing bleeding risk Variable fat depth - Subcutaneous tissue is less consistent here, raising the chance of injecting into muscle Clinical guidance from major medical centres is explicit: Do NOT use the inner thigh. Stick to the front and slightly outer aspect of your upper thigh, roughly the upper third of the leg
After centrifugation at 15,000 g for 50 min, the cell supernatant was incubated with GST beads (Cytiva, Glutathione Sepharose 4B, 17075605) at 4 C for 1.52 h
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