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Step 1: Clean everything Wipe the rubber stoppers on both vials (retatrutide and bacteriostatic water) with separate alcohol swabs
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Analytical verification requires reverse-phase HPLC with UV detection at 214-220 nm to confirm retention time consistency and peak purity above 95%, while mass spectrometry (MALDI-TOF or ESI-MS) can confirm the expected molecular ion at 1419.53 Da and identify any truncation or oxidation products
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