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SDS-PAGE and western blotting Samples were loaded onto 10% SDS-polyacrylamide gels then transferred to 0.45 m PVDF membranes and blocked with 5% skimmed milk [or 5% bovine serum albumin (BSA) for anti-phospho antibodies] in TBS buffer supplemented with 0.1% Tween 20 (TBS-T)
Notes on the estimation of the numerical density of arbitrary profiles: the edge effect
Invasion of transfected CFPAC-1 cells was assessed by counting the numbers of cells that invaded through Matrigel-coated Transwell chambers with 8-m pore size membranes (BD Biosciences)
Cagrilintide: What the Human Evidence Actually Shows Cagrilintides human record is real but narrower than the hype implies, and it splits cleanly into monotherapy and combination
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